poster describing autoclaving biohazardous waste bags and how to properly prepare them

Title: Autoclaving Biohazardous Waste Bags

  • Biohazard bag not overfilled (<3/4 full)
  • Seal Loosely
  • Proper placement of indicator tape
  • Secondary containment
  • Bag is upright

Title:  Biohazard Incident Reporting Matters

Text:  Exposures and spills of materials containing biohazards, including recombinant or synthetic nucleic acids, must be reported to EHS.

Reporting incidents to EHS within 24 hours. or 402.472.4925

  • Animal Bite - Transgenic or pathogen-infected
  • Needle Stick - Biohazard contaminated
  • Biohazard Spill - R/SNA or human pathogen
Image of Biohazard program poster reminding readers to keep an up to date inventory of what is in the freezer

Title:  EHS Biosafety Program

Text:  What's in your freezer?

  • Clean out your freezer while updating your inventory
  • Contact EHS at (402) 472-4925 for assistance with disposal of unwanted or abandoned biological materials
Handout of disposing of garbage.

Title: Disposal of Biohazardous Waste Bags


  • Bag loosely closed
  • Autoclave tape used in "X" pattern
  • Place in tub
  • Use Proper autoclave cycle


  • Carefully remove from autoclave with heat resistant gloves
  • Look for color change on autoclave tape
  • Allow to cool
  • Clean up any spills


  • Place autoclaved bag in BLACK trash bag*
  • Dispose in dumpster

*Black trash bags are provided by user

Image of flourescent materials safety poster

Title:  Fluorescent Materials


Any work with GFP or other fluorescent molecules introduced into microorganisms needs to be registered with the biosafety committee